International Technologist in Cytogenetics ASCP Practice Test

In microscopy, the ability to achieve resolution is fundamentally related to the wavelength of the light used. A shorter wavelength light improves resolution because it allows for the detection of smaller structures within a specimen. This is due to the fact that the resolution of a microscope is defined by its ability to distinguish two points as separate entities. According to the Rayleigh criterion, which is a principle used to determine the resolving power of an optical system, the minimum resolvable distance between two points is directly related to the wavelength of the light being used.

When shorter wavelengths (like ultraviolet light) are employed, the ability to distinguish between two closely spaced objects is enhanced. This is because shorter wavelengths have a higher energy and can interact with smaller features, providing clearer and finer details in the image. Therefore, utilizing a shorter wavelength results in increased resolution, allowing for improved visualization of microscopic details.

In contrast, longer wavelengths, such as those from visible light, result in reduced resolution capabilities because their larger size cannot effectively resolve smaller features. Hence, the relationship between wavelength and resolution is critical in microscopy, making the assertion that a shorter wavelength increases resolution accurate and insightful for understanding this fundamental aspect of microscopy.