24 hours after culture initiation, a single dish from an amniotic fluid turns cloudy. Which cultures should be disposed?

Prepare for the International Technologist in Cytogenetics ASCP exam with comprehensive flashcards and practice questions. Each question is accompanied by hints and explanations. Ace your test with confidence!

In the scenario presented, the focus is on the observation that one specific dish has turned cloudy after 24 hours of culture initiation. This cloudiness is indicative of bacterial contamination, which can compromise the integrity and viability of the cultured cells.

When a single dish from an amniotic fluid culture becomes cloudy, it is crucial to recognize that only that specific culture is contaminated. Therefore, the appropriate action is to dispose of this single cloudy dish only, as it poses a risk of affecting subsequent analyses or results. The other cultures, assuming they do not exhibit similar signs of contamination, can remain intact and are still valid for further examination.

This approach helps maintain the viability of other cultures that may provide important genetic information while ensuring that any contaminated sample is safely discarded to prevent any cross-contamination or inaccurate results in testing.

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