If GTG banded chromosomes appear ghost-like with little visible banding, how should one alter the banding preparation?

When GTG banded chromosomes appear ghost-like with minimal visible banding, decreasing the trypsin time is the appropriate action to enhance the visibility of the bands. This situation often occurs when trypsin digestion has been too prolonged, causing excessive cleavage of chromatin and resulting in the loss of distinct banding patterns. Reducing the trypsin exposure allows for better preservation of the chromosomal architecture, facilitating clearer banding patterns that are crucial for accurate cytogenetic analysis.

The objective in cytogenetics is to achieve an optimal balance in trypsin digestion, ensuring that the chromosomes are adequately banded without losing essential structural details. Properly evaluating the duration of trypsinization is fundamental, as it can directly impact the quality of the cytogenetic preparations.