What banding technique would confirm an inversion of chromosome 9?

C-banding is specifically useful for visualizing the heterochromatin regions of chromosomes, particularly at the centromeres and pericentric areas. This method highlights the constitutive heterochromatin, which can be particularly helpful for identifying structural abnormalities like inversions in chromosomes. Inversions often involve rearrangements that might alter the appearance of heterochromatin, making C-banding a relevant choice for confirming such changes.

G-banding is more commonly used for karyotyping and detecting larger chromosomal abnormalities, as it provides a more general pattern of staining that differentiates various regions of chromosomes based on their DNA composition. While G-banding can sometimes reveal inversions, it is not specifically tailored for identifying the inversion events and may not provide the clarity needed to confirm such structural rearrangements as effectively as C-banding.

NOR banding is utilized to visualize the nucleolar organizing regions on chromosomes, which are typically involved with ribosomal RNA synthesis. Although it can provide information about certain chromosome regions, it is less applicable for confirming inversions as it focuses on specific nucleolar structures rather than the entire chromosomal arrangement.

FISH (Fluorescence In Situ Hybridization) is a technique that utilizes fluorescent probes to bind specific parts of the chromosome