What is the best type of objective to use for photographing a met with optimal results?

The best type of objective to use for photographing a metaphase spread, such as seen in cytogenetics, is the PlanApo 100/1.4 Oil lens. This objective lens is designed to provide high-resolution imaging due to its apochromatic correction, which reduces chromatic aberrations and improves color fidelity. The numerical aperture (NA) of 1.4 allows for greater light gathering, resulting in clearer and more detailed images.

Using an oil immersion lens, such as the 100x objective, enhances the resolution and contrast of the image because the oil has a refractive index closer to that of glass. This minimizes light refraction at the glass-air interface, facilitating better visualization of fine details in the metaphase chromosomes. Consequently, this combination of high magnification and excellent optical performance makes the PlanApo 100/1.4 Oil lens ideal for capturing high-quality photographs of cytogenetic samples.

In contrast, other objective types, while useful in various contexts, do not provide the same level of detail or resolution as the PlanApo 100/1.4 Oil lens. For example, the 40x objective lens may be suitable for initial views or lower magnification needs, but it lacks the