Which technique is associated with increasing band resolution over GTG banding in microscopy?

The technique associated with increasing band resolution over GTG (Giemsa-Trypsin-Giemsa) banding in microscopy is related to the use of decreased trypsin time. By reducing the duration of trypsin digestion, greater detail of the chromosomal bands can be visualized. This is because shorter exposure to trypsin preserves the structural integrity of the chromatin and enhances the visibility of characteristic banding patterns, which are crucial for accurate chromosomal identification and analysis.

Higher magnification, while potentially allowing for finer details of the bands to be seen, does not inherently improve the resolution of the bands themselves; it simply enlarges the size of what is already present. Fluorescent probes are a different technique used primarily in fluorescence in situ hybridization (FISH) and serve a distinct purpose, focusing on specific regions rather than enhancing banding resolution. Increasing chromosome condensation generally could lead to a more compact structure and may obscure finer bands rather than resolving them, as it can potentially make individual bands indistinguishable.