Why is it important to adjust lighting after changing to a higher magnification objective lens?

When using a higher magnification objective lens, it is essential to adjust the lighting because higher magnification requires more light for proper detail visibility. At increased magnification, the lens gathers light from a narrower field of view, which can result in a dimmer image. This is due to the way lenses concentrate light; as magnification increases, the effective area of the specimen being viewed decreases, thus reducing the amount of light hitting the sensor or observer's eye.

Adequate illumination is crucial to ensure that details of the specimen can be observed clearly, preventing the image from appearing too dark or unclear. In microscopy, if the illumination is not adjusted accordingly when switching to a higher magnification, the observer may miss critical details that are essential for analysis or diagnosis. Therefore, adjusting the lighting appropriately enhances visibility and allows for better observation of fine features in the specimen being examined.